| 1 | Maxwell, Nyoka (1997) | Extreme Velocity Track Club | 24.15 Q | +0.7 | 1v2 |
| 2 | Jacques, Farah (1990) | Perfmax-Racing | 24.20 Q | +0.8 | 1v3 |
| 3 | Green, Deondra (2000) | Kilomaîtres De Lasalle | 24.39 Q | +2.0 | 1v1 |
| 4 | Léger, Catherine (2000) | Saint-Laurent Sélect | 24.78 Q | +0.7 | 2v2 |
| 5 | Jackson, Audrey (1998) | Saint-Laurent Sélect | 24.87 Q | +2.0 | 2v1 |
| 6 | Allen-Destin, Angélina (2004) | Vainqueurs Plus | 25.28 Q | +0.8 | 2v3 |
| 7 | Desormes, Laurie-Ann (2003) | Tarus | 25.42 q | +0.7 | 3v2 |
| 8 | Béliveau, Émy (1995) | Saint-Laurent Sélect | 25.64 q | +0.8 | 3v3 |
| 9 | Gould, Stephanie (1997) | Ottawa Lions | 26.39 q | +2.0 | 3v1 |
| 10 | Guipi-Bopala, Gloriana (1997) | Corsaire-Chaparral | 26.77 q | +2.0 | 4v1 |
| 11 | Myre-Bourgault, Marianne (1994) | Rive-Sud | 26.80 q | +0.7 | 4v2 |
| 12 | Rocheleau, Myriam (1999) | C. A. Université Laval | 27.09 q | +0.8 | 4v3 |
| 13 | Charles, Lily-Rose (2004) | Tarus | 27.30 q | +0.7 | 5v2 |
| 14 | Dubé, Élisabeth (1995) | Saint-Laurent Sélect | 27.47 q | +2.0 | 5v1 |
| 15 | Lavoie, Gabrielle (2002) | Zénix De La Mauricie | 27.51 q | +0.8 | 5v3 |
| 16 | Audet, Alexe (1993) | Corsaire-Chaparral | 27.81 q | +2.0 | 6v1 |
| 17 | Rouleau, Andréa (2003) | Mustangs | 28.81 | +2.0 | 7v1 |
| 18 | Lepage, Rosalie (2002) | Lanaudière-Olympique | 29.75 | +0.7 | 6v2 |
| 19 | Boutilier, Shanna (1996) | Ottawa Lions | 30.10 | +2.0 | 8v1 |
| 20 | Bonneau, Louise (1969) | Vainqueurs Plus | 30.44 | +0.8 | 6v3 |
| --- | Bessette, Roxanne (1996) | C. A. Université Laval | DNF | +0.7 | v2 |
| --- | Fortin-Bélanger, Axele (2000) | Filoup | DNS | +0.8 | v3 |